LOCUS       40924_42389        3846 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT7XbG2-AcGFP1 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Kikawada T, Kanamori Y, Saito A, Watanabe M, Okuda T.
  TITLE     Expression vector for Xenopus oocyte
  JOURNAL   Published Only in Database (2006)
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Kikawada T, Kanamori Y, Saito A, Watanabe M, Okuda T.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-MAR-2006) Takahiro Kikawada, National Institute of 
            Agrobiological Sciences; Ohwashi 1-2, Tsukuba 305-8634, Japan 
            (E-mail:kikawada@affrc.go.jp, Tel:81-29-838-6087)
REFERENCE   3  (bases 1 to 3846)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3846)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2006)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-MAR-2006) Takahiro Kikawada, National Institute of 
            Agrobiological Sciences"; volume: " Ohwashi 1-2, Tsukuba 305-8634, 
            Japan (E-mail:kikawada@affrc.go.jp, Tel"; pages: "81-29-838-6087"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           659..687
                     /note="Xenopus beta-globin 5'-UTR, sub-cloned from
                     pXbG-ev1"
     misc_feature    687..699
                     /note="contains multiple cloning site (BgI II, EcoR V, EcoR
                     I)"
     CDS             714..1430
                     /label=AcGFP1
                     /note="Aequorea coerulescens GFP"
     3'UTR           1457..1582
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     promoter        complement(1658..1676)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1697..1713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1721..1737)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1745..1775)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1790..1811)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2099..2687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2861..3718)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3719..3823)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 gctcagaata aacgctcaac tttggcagat ctgatatcga attcaaaccc gggatggtga
      721 gcaagggcgc cgagctgttc accggcatcg tgcccatcct gatcgagctg aatggcgatg
      781 tgaatggcca caagttcagc gtgagcggcg agggcgaggg cgatgccacc tacggcaagc
      841 tgaccctgaa gttcatctgc accaccggca agctgcctgt gccctggccc accctggtga
      901 ccaccctgag ctacggcgtg cagtgcttct cacgctaccc cgatcacatg aagcagcacg
      961 acttcttcaa gagcgccatg cctgagggct acatccagga gcgcaccatc ttcttcgagg
     1021 atgacggcaa ctacaagtcg cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc
     1081 gcatcgagct gaccggcacc gatttcaagg aggatggcaa catcctgggc aataagatgg
     1141 agtacaacta caacgcccac aatgtgtaca tcatgaccga caaggccaag aatggcatca
     1201 aggtgaactt caagatccgc cacaacatcg aggatggcag cgtgcagctg gccgaccact
     1261 accagcagaa tacccccatc ggcgatggcc ctgtgctgct gcccgataac cactacctgt
     1321 ccacccagag cgccctgtcc aaggacccca acgagaagcg cgatcacatg atctacttcg
     1381 gcttcgtgac cgccgccgcc atcacccacg gcatggatga gctgtacaag tgacaattcg
     1441 gatctggtta ccactaaacc agcctcaaga acacccgaat ggagtctcta agctacataa
     1501 taccaactta cactttacaa aatgttgtcc cccaaaatgt agccattcgt atctgctcct
     1561 aataaaaaga aagtttcttc acattctaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1621 accccccccc cccccccccg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     1681 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1741 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1801 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1861 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1921 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1981 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2521 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2701 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2761 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2821 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2881 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2941 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3001 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3061 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3121 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3181 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3241 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3301 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3361 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3421 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3481 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3541 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3601 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3661 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3721 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3781 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3841 tgccac
//
